silver stain plustm kit  (Bio-Rad)

Journal: Biochemistry

Article Title: Allosteric Inhibition of the Epidermal Growth Factor Receptor

doi: 10.1021/acs.biochem.0c00978

Figure Lengend Snippet: EGFR645–1186 is a model for the interaction of E1S with full length EGFR in cells. (A) Silver Stain blots illustrating the relative concentration of EGFR fragments EGFR645–1186, EGFR672–1186, or EGFR1–621 (0.15 μM) incubated with 0 or 100 equivalents DBE1S or BDE1S for 2 h, then irradiated (365 nm,10 min). Samples were resolved by SDS-PAGE (10% polyacrylamide) and stained with Silver Stain PlusTM (BioRad) (B) Immunoblots illustrating the relative amounts of biotinylated fragments EGFR645–1186, EGFR672–1186, or EGFR1–621 post- incubation with 0, 1, 10, 50 or 100 equivalents of DBE1S or BDE1S for 2 h, irradiated, resolved and immunoblotted using an anti-Biotin antibody. See also Figure S3.

Article Snippet: Samples were resolved by SDS-PAGE (10% polyacrylamide) and stained with Silver Stain PlusTM (BioRad) (B) Immunoblots illustrating the relative amounts of biotinylated fragments EGFR645–1186, EGFR672–1186, or EGFR1–621 post- incubation with 0, 1, 10, 50 or 100 equivalents of DB E1 S or BD E1 S for 2 h, irradiated, resolved and immunoblotted using an anti-Biotin antibody.

Techniques: Silver Staining, Concentration Assay, Incubation, Irradiation, SDS Page, Staining, Western Blot